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Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray

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Tutor: WangZhenYu
School: Harbin Institute of Technology
Course: Biochemical Engineering
Keywords: apple polyphenol,purification,γ-ray,immune system
CLC: S661.1
Type: Master's thesis
Year:  2008
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Abstract:
Apple polyphenol was selected as an objective of study, and extraction technology was optimizated, the crude apple polyphenol was separated and purificated by macroporous resin and the anti-radiation function of the apple polyphenol was studied further in this research.Using ultrasound-assisted extraction method, apple polyphenols of Gala was extracted, and using response surface analysis extraction process was optimized, at the end the best extraction process of apple polyphenol was obtained: ultrasonic time 37.51 min, the temperature 41.18℃, acetone concentration of 69.36 percent, the ratio of 1:5.21. In this condition, theoretical yeild of the apple polyphenol was up to 1.170 mg/g. After ultrasound-assisted extraction and ethanol purify settlement, apple polyphenol solution was still crude, and apple polyphenol powder with the taste of apple could be obtained by macroporous resin AB-8. FC determination of the purity was up to 79.5 percent, the yield up to 65.1 percent. There were not n-hexane, benzene, toluene and xylene, o-xylene, styrene and other organic solvent residue in the purification of apple polyphenol by the headspace gas chromatograph.Respectively using DPPH method, methyl-purple method, auto-oxidation of pyrogall method, DPPH radical、O2- and ?OH were scavenged by the purification of apple polyphenol. The experimental results showed that: DPPH radical, O2- and OH were significantly scavenged by the purification of apple polyphenol, and the IC50 value was 0.0034 mg/mL, 2.86mg/mL, 0.75mg/mL.Constructed radiation model and determined 5 Gyγ-ray as the best dose of the immune system radiation damage. Compared with the control group, added the purification of apple polyphenols before irradiation, the carbon clearance index、the serum hemolysin、the swelling degree of the paw、and the index of spleen had increased, 1000 mg/kg/d was significantly in statistics. And the content of MDA and the activity of SOD and GSH-Px in the liver of irrated mice was markedly improved, and 1000 mg/kg/d was statistical significance.
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